NOTCH3 Antibody

SKU: orb19112

Description

Goat polyclonal antibody to NOTCH3

Research Area

Cardiovascular Disease, Developmental Biology, Epigenetics, Neuroscience

Images & Validation

• Tested Applications: ELISA, FC, IHC
• Dilution Range: ELISA: 1:16000, IHC-P: 5 μg/ml
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave approx 60+70kDa bands in human brain lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 244kDa according to NP_000426.1. The 60+70kDa bands were successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

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Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: NOTCH3
• Protein Sequence: QLGPQPEVTPKRQ
• Molecular Weight: 244
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti NOTCH3 antibody, anti Notch homolog 3 (Drosophila) antibody, anti HGNC:7883 antibody, anti CADASIL antibody, anti CASIL antibody, anti Notch (Drosophila) homolog 3 antibody, anti Notch homolog 3 antibody

Quick Database Links

Gene Symbol

NOTCH3

NCBI Gene ID

4854