NRAS Antibody

SKU: orb19888

Description

Goat polyclonal antibody to NRAS

Research Area

Cancer, Epigenetics

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:64000, WB: 0.3-1μg/ml
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Small Intestine shows staining of cytoplasm and membrane of enterocytes. Recommended concentration, 2-3ug/ml.WB: Approx. 21kDa band observed in Human lysates of cell line A431 (calculated MW of 21.2kDa according to NP_002515.1). In transfected HEK293 transiently expressing NRAS bands of approx. 21kDa and 25kDa are observed. These bands are not observed in the non-transfected HEK293. Recommended concentration: 0.01-0.03 μg/ml.

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Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: NRAS
• Protein Sequence: RMKKLNSSDDGTQ
• Molecular Weight: 21.2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti NRAS antibody, anti neuroblastoma RAS viral (v-ras) oncogene homolog antibody, anti N-ras antibody, anti NRAS1 antibody, anti N-ras protein part 4 antibody, anti v-ras neuroblastoma RAS viral oncogene homolog antibody

Quick Database Links

Gene Symbol

NRAS

NCBI Gene ID

4893