PAX3 Antibody

SKU: orb18763

Description

Goat polyclonal antibody to PAX3

Research Area

Cancer, Developmental Biology, Epigenetics, Stem Cells

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:32000, WB: 0.3-1 μg/ml, IHC-P: 4.5 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.IHC: In paraffin embedded Human Oesophagus shows nuclear staining of cells in the Squamous Epithelium. Recommended concentration, 3ug/ml.WB: Preliminary experiments gave an approx 30-35kDa band in Human Kidney lysates at 1 μg/ml. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted sizes of approx. 24-58kDda according to NP_000429.2; NP_039230.1; NP_852122;.1 NP_852123.1; NP_852124.1; NP_852125.1; NP_852126.1. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

  Read more...

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: PAX3
• Protein Sequence: TTLAGAVPRMM
• Molecular Weight: 53.0; 24.1; 22.7; 53.5; 56.0; 44.8; 45.2; 53.3
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti PAX3 antibody, anti WS1 antibody, anti CDHS antibody, anti HUP2 antibody, anti paired box gene 3 (Waardenburg syndrome 1) antibody, anti paired domain gene 3 antibody, anti paired box homeotic gene 3 (Waardenburg syndrome 1) antibody, anti paired box 3 antibody, anti MGC120381 antibody, anti MGC120382 antibody, anti MGC120383 antibody, anti MGC120384 antibody, anti MGC134778 antibody, anti paired box homeotic gene 3 antibody, anti paired domain gene HuP2 antibody

Quick Database Links

Gene Symbol

PAX3

NCBI Gene ID

5077