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RNF7 Antibody

SKU: orb20424

Description

Goat polyclonal antibody to RNF7

Research Area

Epigenetics

Images & Validation

Tested ApplicationsELISA, IHC, WB
Dilution RangeELISA: 1:8000, WB: 1-3 μg/ml, IHC-P: 5 μg/ml
ReactivityHuman
Predicted ReactivityMouse
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 11kDa band observed in Human Human Heart lysates (calculated MW of 12.7kDa according to NP_055060.1). Recommended concentration: 1-3 μg/ml. An additional band of 28kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.

Key Properties

HostGoat
ClonalityPolyclonal
TargetRNF7
Protein SequenceDACLRCQAENKQE
Molecular Weight12.7; 9.64
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti RNF7 antibody, anti ring finger protein 7 antibody, anti CKBBP1 antibody, anti ROC2 antibody, anti SAG antibody, anti OTTHUMP00000180645 antibody, anti regulator of cullins 2 antibody, anti sensitive to apoptosis, zinc RING finger protein SAG, regulator of cullins 2 antibody, anti zinc RING finger protein SAG antibody

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Quality Guarantee

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NCBI Gene Details

No NCBI Gene data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

Select a size below

100 μg
$ 500.00
Choose Conjugation or Carrier Free Version
Free Secondary Antibody (20 ul)0/0

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