Goat anti-UBE2C / UBCH10 Antibody

SKU: orb18433

Description

Goat polyclonal antibody to UBE2C

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, FC, IF, IP, WB
• Dilution Range: ELISA: 1:32000, WB: 1-3 μg/ml, IHC-P: 10ug/ml
• Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approximately 20KDa band was detected in mitotic (i.e Nocodazole arrested) HeLa whole cell lysates Recommended for use at 1-3 μg/ml. Immunoprecipitation: 20KDa band precipitated from mitotic HeLa whole cell lysates using protein-G dynabeads. Immunofluorescence: Stained mitotic and late G2 Hela cells, with very faint or no signal in G1 and S phase cels (fixed using paraformaldehyde, primary concentration 2.5 μg/ml, using FITC anti-Goat)

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Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: UBE2C / UBCH10
• Protein Sequence: QETYSKQVTSQEP
• Molecular Weight: 19.7; 17.4; 16.7; 15.8; 14.3
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti UBE2C antibody, anti UBCH10 antibody, anti dJ447F3.2 antibody, anti ubiquitin-conjugating enzyme E2C antibody, anti ubiquitin-protein ligase C antibody, anti ubiquitin carrier protein E2-C antibody, anti cyclin-selective ubiquitin carrier protein antibody, anti mitotic-specific ubiquitin-conjugating enzyme antibody, anti RP3-447F3.1 antibody

Quick Database Links

Gene Symbol

UBE2C / UBCH10

NCBI Gene ID

11065