USP16 Antibody

SKU: orb20383

Description

Goat polyclonal antibody to USP16

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA
• Dilution Range: ELISA: 1:32000, WB: 0.5 μg/ml
• Predicted Reactivity: Bovine, Canine, Equine, Human, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave bands at approx 50kDa and 26kDa in lysates of cell lines HEK293, A549 and HeLa after 0.5 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 93.5kDa according to NP_006438.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

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Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: USP16
• Protein Sequence: NVEWNICQDCKTDNK
• Molecular Weight: 93.6; 93.5
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti USP16 antibody, anti ubiquitin specific peptidase 16 antibody, anti UBP-M antibody, anti deubiquitinating enzyme 16 antibody, anti ubiquitin carboxyl-terminal hydrolase 16 antibody, anti ubiquitin specific protease 16 antibody, anti ubiquitin thiolesterase 16 antibody, anti ubiquitin-specific processing protease 16 antibody

Quick Database Links

Gene Symbol

USP16

NCBI Gene ID

10600