VPS35/MEM3 Antibody

SKU: orb18631

This product can be custom made

Description

Goat polyclonal antibody to VPS35

Research Area

Epigenetics, Infectious Diseases, Neuroscience

Images & Validation

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Item 1 of 6

Tested Applications	ELISA, IF, IHC, WB
Dilution Range	ELISA: 1:128000, WB: 1.0-3.0 μg/ml, IHC-P: 5-8 μg/ml
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Bovine
Application Notes	ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.IHC: In paraffin embedded Human Testis, shows positive staining in Leydig cells, and cytoplasmic staining (dot-like) of some epithelial cells of the epididymis and of the rete testis.WB: Approx 90kDa band observed in Human Brain and Testis lysates (predicted MW of 91kDa according to NP_060676.2). Recommended for use at 1-3 μg/ml.Experiment Notes: Immunoflurescence: Staining of VPS35 (red) in human melanoma cells 1205LU: Please see Scott KL et al.; Nature. 2009 Jun 25;459(7250):1085-90.; PMID: 19553991.

Key Properties

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Host	Goat
Clonality	Polyclonal
Target	VPS35 / MEM3
Protein Sequence	SPESEGPIYEGLIL
Molecular Weight	91.7
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration	< p>1-3 μg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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anti VPS35 antibody, anti MEM3 antibody, anti FLJ10752 antibody, anti FLJ13588 antibody, anti FLJ20388 antibody, anti DKFZp434E1211 antibody, anti DKFZp434P1672 antibody, anti vacuolar protein sorting 35 (yeast) antibody, anti maternal-embryonic 3 antibody, anti vacuolar protein sorting 35 homolog (S. cerevisiae) antibody, anti vacuolar protein sorting 35 antibody

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