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WNT3 Antibody

SKU: orb19254

Description

Goat polyclonal antibody to WNT3

Research Area

Cancer, Developmental Biology, Epigenetics, Neuroscience, Signaling Pathways, Wnt

Images & Validation

Tested ApplicationsELISA, IHC, WB
Dilution RangeELISA: 1:16000, WB: 1-3 μg/ml, IHC-P: 2-4 μg/ml
ReactivityHuman
Predicted ReactivityCanine, Mouse, Rat
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:2000.IHC: In paraffin embedded Human Breast shows strong fibrous staining in myoepithelial cells. Recommended concentration, 3-5ug/ml.WB: Approx 38kDa band observed in Human Brain lysates (calculated MW of 39.6kDa according to NP_110380.1). Recommended concentration: 1-3 μg/ml.

Key Properties

HostGoat
ClonalityPolyclonal
TargetWNT3
Protein SequenceCGRGHNTRTEKRKEK
Molecular Weight39.6
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti WNT3 antibody, anti wingless-type MMTV integration site family, member 3 antibody, anti INT4 antibody, anti MGC131950 antibody, anti MGC138321 antibody, anti MGC138323 antibody, anti WNT-3 proto-oncogene protein precursor antibody

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Quality Guarantee

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NCBI Gene Details

No NCBI Gene data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

Select a size below

100 μg
$ 500.00
Choose Conjugation or Carrier Free Version
Free Secondary Antibody (20 ul)0/0

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