GPX1 Rabbit Polyclonal Antibody

SKU: orb5344

Description

GPX1 Rabbit Polyclonal Antibody

Research Area

Antioxidants, Metabolism, Cancer Research, Mitochondrial Markers, Cell Biology, Mitochondrial, Cell Biology, Oxidative Stress

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test, ELISA=1:5000-10000
• Reactivity: Human, Mouse, Rat, Sheep
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Porcine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from mouse Glutathione Peroxidase 1 (51-150/201aa)
• Target: GPX1
• Molecular Weight: 22 kDa
• Purification: affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Glutathione peroxidase 1; Cellular glutathione peroxidase; GPx 1; GPx-1; GPX1; GPX1_HUMAN; GSHPx-1; GSHPX1; MGC14399; MGC88245.

Blank control: U937. Primary Antibody (green line): Rabbit Anti-GPX1 antibody (orb5344), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at PBST. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lane 1: Mouse Liver tissue lysates, Lane 2: Mouse Spleen tissue lysates, Lane 3: Mouse Kidney tissue lysates, Lane 4: Rat Liver tissue lysates, Lane 5: Rat Heart tissue lysates, Lane 6: Rat Spleen tissue lysates, Lane 7: Rat Kidney tissue lysates, Primary: Anti-GPX1 (orb5344) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 22 kDa, Observed band size: 22 kDa.

Sample: Spleen (Mouse) Lysate at 40 ug, Primary: Anti-GPX1 (orb5344) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 22 kD, Observed band size: 22 kD.

Quick Database Links

Gene Symbol

GPX1

UniProt

P11352