GRB10 Antibody (N-term)

SKU: orb134951

Description

Rabbit polyclonal to GRB10. Adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. Binds to, and suppress signals from, activated receptors tyrosine kinases, including the insulin (INSR) and insulin-like growth factor (IGF1R) receptors. The inhibitory effect can be achieved by 2 mechanisms: interference with the signaling pathway and increased receptor degradation. Delays and reduces AKT1 phosphorylation in response to insulin stimulation. Blocks association between INSR and IRS1 and IRS2 and prevents insulin-stimulated IRS1 and IRS2 tyrosine phosphorylation. Recruits NEDD4 to IGF1R, leading to IGF1R ubiquitination, increased internalization and degradation by both the proteasomal and lysosomal pathways. May play a role in mediating insulin-stimulated ubiquitination of INSR, leading to proteasomal degradation. Negatively regulates Wnt signaling by interacting with LRP6 intracellular portion and interfering with the binding of AXIN1 to LRP6. Positive regulator of the KDR/VEGFR-2 signaling pathway. May inhibit NEDD4-mediated degradation of KDR/VEGFR-2.

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics,Crown Antibodies;Primary Antibodies;Signal Transduction

Images & Validation

• Tested Applications: WB
• Dilution Range: WB: 1:1000
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: Synthetic Peptide
• Target: GRB10
• Molecular Weight: 67231
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti GRB10 antibody, anti GRBIR antibody, anti KIAA0207 antibody, anti Growth factor receptor-bound protein 10 antibody, anti Growth factor receptor-bound protein 10 antibody, anti GRB10 adapter protein antibody, anti Growth factor receptor-bound protein 10 antibody, anti Insulin receptor-binding protein Grb-IR antibody

GRB10 Antibody (N-term) western blot analysis in A549, MDA-MB453, U251 cell line lysates (35 ug/lane). This demonstrates the GRB10 antibody detected the GRB10 protein (arrow).

Quick Database Links

Gene Symbol

GRB10

UniProt

Q13322

RefSeq (Protein):NP_001001550.1, NP_001001549.1, NP_001001555.1, NP_005302.3