Growth Hormone Antibody / GH1

SKU: orb2643299

Description

Pituitary growth hormone (GH) plays a crucial role in stimulating and controlling the growth, metabolism and differentiation of many mammalian cell types by modulating the synthesis of multiple mRNA species. These effects are mediated by the binding of GH to its membrane-bound receptor, GHR, and involve a phosphorylation cascade that results in the modulation of numerous signaling pathways. GH is synthesized by acidophilic or somatotropic cells of the anterior pituitary gland. Anti-GH is a useful marker in classification of pituitary tumors and the study of pituitary disease (acromegaly).

Images & Validation

• Tested Applications: IHC-P
• Dilution Range: Immunohistochemistry (FFPE): 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: GH/3155
• Immunogen: A full-length of human Growth Hormone protein was used as the immunogen for the Growth Hormone antibody.
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This Growth Hormone antibody is available for research use only.
• Disclaimer: For research use only

IHC testing of FFPE human pituitary with Growth Hormone antibody (clone GH/3155). Required HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min.

IHC testing of FFPE human pituitary with Growth Hormone antibody (clone GH/3155). Required HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min.

IHC testing of FFPE human pituitary with Growth Hormone antibody (clone GH/3155). Required HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using Growth Hormone antibody (clone GH/3155). These results demonstrate the foremost specificity of the GH/3155 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free recombinant Growth Hormone antibody (clone GH/3155) as confirmation of integrity and purity.

Quick Database Links

UniProt

P01241