GRP78 Antibody: RPE

SKU: orb148030

Description

Mouse monoclonal to GRP78 (RPE). GRP78 is a ubiquitously expressed, 78-kDa glucose- regulated protein, and is commonly referred to as an immunoglobin chain binding protein (BiP). The BiP proteins are categorized as stress response proteins because they play an important role in the prope..

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics,Cancer | Heat Shock | Cell Signaling | Protein Trafficking | Chaperone Proteins | Organelle Markers

Images & Validation

• Tested Applications: ICC, IF, IHC, WB
• Dilution Range: WB (1:2000), ICC/IF (1:100)
• Reactivity: Bovine, Frog, Fungi, Hamster, Human, Monkey, Mouse, Plant, Rabbit, Rat
• Application Notes:
  0.5 µg/ml of SMC-196 was sufficient for detection of Grp78 in 10 µg of HeLa cell lysate by ECL immunoblot analysis.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 6H4-2G7
• Immunogen: His-tagged human GRP78
• Target: GRP78
• Molecular Weight: 78kDa
• Conjugation: RPE

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 95.64mM Phosphate, 2.48mM MES and 2mM EDTA
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

78 kDa glucose regulated protein antibody, 78 kDa glucose-regulated protein antibody, AL022860 antibody, AU019543 antibody, BIP antibody, D2Wsu141e antibody, D2Wsu17e antibody, Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 antibody, Endoplasmic reticulum lumenal antibody, Ca2+ binding protein grp78 antibody, FLJ26106 antibody, Glucose Regulated Protein 78kDa antibody, GRP 78 antibody, GRP-78 antibody, GRP78_HUMAN antibody, Heat shock 70 kDa protein 5 antibody, Heat Shock 70kDa Protein 5 antibody, HSCe70 antibody, HSPA 5 antibody, HSPA5 antibody, Immunoglobulin Heavy Chain Binding Protein antibody, Immunoglobulin heavy chain-binding protein antibody, mBiP antibody, MIF2 antibody, Sez7 antibody

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GRP78 Monoclonal Antibody, Clone 6H4-2G7. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Endoplasmic Reticulum, Endoplasmic Reticulum Lumen. Magnification: 60X.

Western Blot analysis of Human recombinant cell lysate showing detection of GRP78 protein using Mouse Anti-GRP78 Monoclonal Antibody, Clone 6H4.2G7. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:1000.

Quick Database Links

Gene Symbol

GRP78

UniProt

P20029

NCBI Gene ID

14828

RefSeq (Protein):NP_001156906.1