GRP78 (Bip) Antibody

SKU: orb1822422

Description

Rabbit Anti-Human GRP78 (Bip) Polyclonal

Research Area

,Cancer | Heat Shock | Cell Signaling | Protein Trafficking | Chaperone Proteins | Tags and Cell Markers | Organelle Markers

Images & Validation

• Tested Applications: ELISA, ICC, IF, IHC, WB
• Dilution Range: WB (1:2000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
• Reactivity: Canine, Drosophila, Hamster, Human, Mouse, Rat
• Application Notes:
  0.5 µg/ml of SPC-180 was sufficient for detection of Grp78 in 10 µg of rat tissue lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Full length human GRP78 (Bip) his tagged at the N terminus
• Target: GRP78
• Molecular Weight: 78kDa
• Conjugation: Unconjugated

Storage & Handling

• Storage: -20°C
• Buffer/Preservatives: PBS, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

78 kDa glucose regulated protein Antibody, 78 kDa glucose-regulated protein Antibody, AL022860 Antibody, AU019543 Antibody, BIP Antibody, D2Wsu141e Antibody, D2Wsu17e Antibody, Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 Antibody, Endoplasmic reticulum lumenal Antibody, Ca2+ binding protein grp78 Antibody, FLJ26106 Antibody, Glucose Regulated Protein 78kDa Antibody, GRP 78 Antibody, GRP-78 Antibody, GRP78_HUMAN Antibody, Heat shock 70 kDa protein 5 Antibody, Heat Shock 70kDa Protein 5 Antibody, HSCe70 Antibody, HSPA 5 Antibody, HSPA5 Antibody, Immunoglobulin Heavy Chain Binding Protein Antibody, Immunoglobulin heavy chain-binding protein Antibody, mBiP Antibody, MIF2 Antibody, Sez7 Antibody

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GRP78 (Bip) Polyclonal Antibody. Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-GRP78 (Bip) Polyclonal Antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-GRP78 (Bip) Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

Western blot analysis of Human Glucose deprived glia cell lysates showing detection of GRP78 protein using Rabbit Anti-GRP78 Polyclonal Antibody. Primary Antibody: Rabbit Anti-GRP78 Polyclonal Antibody at 1:1000.

Quick Database Links

Gene Symbol

GRP78

UniProt

P11021

NCBI Gene ID

3309

RefSeq (Protein):NP_005338.1