GRP94 Antibody

SKU: orb1822510

Description

Rat Anti-Chicken GRP94 Monoclonal IgG2a

Research Area

,Cancer | Heat Shock | Cell Signaling | Protein Trafficking | ER Proteins | Tags and Cell Markers | Organelle Markers | Cancer | Cancer Metabolism | Response to Hypoxia | Cell Signaling | Metabolism | Metabolism processes | Hypoxia

Images & Validation

• Tested Applications: FC, ICC, IF, IP, WB
• Dilution Range: WB (1:2000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
• Reactivity: Bovine, Canine, Equine, Frog, Gallus, Guinea pig, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep
• Application Notes:
  0.5 µg/ml of SMC-105 was sufficient for detection of Grp94 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.

Key Properties

• Host: Rat
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: 9G10
• Immunogen: Purified Grp94 isolated from chicken oviducts
• Target: GRP94
• Molecular Weight: 98kDa
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS pH 7.2, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HSP90B1 Antibody, GP96 Antibody, TRA1 Antibody, ECGP Antibody, 94 kDa glucose regulated protein antibody, 94 kDa glucose-regulated protein antibody, ECGP antibody, Endoplasmin antibody, Endothelial cell (HBMEC) glycoprotein antibody, ENPL_HUMAN antibody, Glucose regulated protein 94kDa antibody, gp96 antibody, gp96 homolog antibody, GRP 94 antibody, GRP-94 antibody, Heat shock protein 90 kDa beta member 1 antibody, heat shock protein 90kDa beta (Grp94), member 1 antibody, Heat shock protein, 90 kDa, beta, 1 antibody, HSP90B1 antibody, Stress inducible tumor rejection antigen GP96 antibody, TRA1 antibody, tumor rejection antigen (gp96) 1 antibody, Tumor rejection antigen 1 antibody, Tumor rejection antigen gp96 antibody, Tumor rejection antigen-1 (gp96) antibody

Immunocytochemistry/Immunofluorescence analysis using Rat Anti-GRP94 Monoclonal Antibody, Clone 9G10. Tissue: Heat Shocked cervical cancer cells (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-GRP94 Monoclonal Antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rat (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-GRP94 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

Immunocytochemistry/Immunofluorescence analysis using Rat Anti-GRP94 Monoclonal Antibody, Clone 9G10. Tissue: Heat Shocked cervical cancer cells (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-GRP94 Monoclonal Antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rat (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-GRP94 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of GRP94 protein using Rat Anti-GRP94 Monoclonal Antibody, Clone 9G10. Primary Antibody: Rat Anti-GRP94 Monoclonal Antibody at 1:1000. Secondary Antibody: HRP Goat Anti-Rat.

Quick Database Links

Gene Symbol

GRP94

UniProt

P14625

NCBI Gene ID

7184

RefSeq (Protein):NP_003290.1