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Description
Research Area
,Apoptosis,Cancer,Cell Cycle,Immunology,Neuroscience,Signal Transduction,Stem Cell
Images & Validation
−Item 1 of 5
| Tested Applications | IF, IHC, IP, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | A synthetic peptide of human GSK3B |
| Target | GSK3B |
| Molecular Weight | Observed: 46kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−GSK3B, GSK-3 beta, GSK3 beta, GSK3beta, GSK3beta isoform, TPKI, GSK 3beta, Tau protein kinase I, ZGSK-3beta
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Western blot analysis of extracts of mouse brain, using GSK3B antibody (orb1258304) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human mammary cancer using GSK3B antibody (orb1258304) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using GSK3B antibody (orb1258304) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using GSK3B antibody (orb1258304) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of C6 cells using GSK3B antibody (orb1258304) at dilution of 1:100. Blue: DAPI for nuclear staining.
Quick Database Links
Gene Symbol
GSK3B
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
IP
Immunoprecipitation
GSK3B Antibody (orb1258304)
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