H2AFX Antibody (N-term)

SKU: orb106887

Description

Mouse monoclonal to H2AFX. Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Cancer,Product Categories/Products/Antibodies/Primary Antibodies,Cancer/DNA Damage,Epigenetics,Infectious Diseases,Crown Antibodies;Primary Antibodies;Catalog Updated;Viral process;Host-virus interaction

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB: 1:1000
• Reactivity: Human
• Predicted Reactivity: Mouse, Zebrafish

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 980CT1.5.5
• Immunogen: Synthetic Peptide
• Target: H2AX
• Molecular Weight: 15145
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti H2AFX antibody, anti H2AX antibody, anti Histone H2A.x antibody

Quick Database Links

Gene Symbol

H2AX

UniProt

P16104

RefSeq (Protein):NP_002096.1