HA Tag Antibody

SKU: orb1927582

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

,Crown Antibodies;Primary Antibodies;Antibody Collections;Tag Antibodies

Images & Validation

• Tested Applications: ChIP, IF, WB
• Dilution Range: IF: 1:10~50, CHIP: 1:100, WB: 1:1000, WB: 1:1000, WB: 1:1000

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB0411
• Target: KLH conjugated synthetic peptide encoding HA tag (CYPYDVPDYAYPYDVPDYA) was used as antigen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Glutathione S-transferase

Western blot analysis of anti-HA tag pab in HA-tagged recombinant protein bacterial lysate.

ChIP was performed with 35S: HATCL1 plants using anti-HA antibodies. Rabbit preimmune serum was used as a mock control. Primer sets specific for the first intron or the 3-UTR region of GL1 were used in PCR reactions.

Western blot analysis of Tag-HA. 2x Antibody (arrow) using rabbit polyclonal. 293T/17 cell lysates either nontransfected (Lane 1) or transiently transfected (Lane 2) with 41kDa HA tagged vector.

GTPase activity of dynamin-2 is required for endocytosis of cell-surface tTG. MRC-5 fibroblasts were transiently transfected with either wild-type (wt) or a GTPase-deficient dynamin-2 mutant (K44A) with a hemagglutinin (HA) tag.

Western blot analysis of lysate from 12 tag recombinant protein cell line, using Tag-HA. 2x Antibody. Diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35 ug.

Quick Database Links

Gene Symbol

KLH conjugated synthetic peptide encoding HA tag (CYPYDVPDYAYPYDVPDYA) was used as antigen.