HADHA Antibody (C-term)

SKU: orb1925236

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

,Crown Antibodies;Primary Antibodies;Cancer;Cardiovascular;Metabolism;Signal Transduction

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution Range: WB: 1:2000, WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB20987
• Target: This HADHA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 737-763 amino acids from the C-terminal region of human HADHA.
• Molecular Weight: 83000 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Trifunctional enzyme subunit alpha, mitochondrial, 78 kDa gastrin-binding protein, TP-alpha, Long-chain enoyl-CoA hydratase, Long chain 3-hydroxyacyl-CoA dehydrogenase, HADHA, HADH

HADHA Antibody (C-term) western blot analysis in A431 cell line lysates (35 ug/lane). This demonstrates the HADHA antibody detected the HADHA protein (arrow).

HADHA Antibody (C-term) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HADHA Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

All lanes: Anti-HADHA Antibody (C-term) at 1:1000 dilution. Lane 1: Hela whole cell lysate. Lane 2: Jurkat whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 83 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-HADHA Antibody (C-term) at 1:2000 dilution. Lane 1: Hela whole cell lysate. Lane 2: 293T whole cell lysate. Lane 3: A431 whole cell lysate. Lane 4: K562 whole cell lysate. Lane 5: Mouse heart lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/15000 dilution.Observed band size: 83KDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

This HADHA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 737-763 amino acids from the C-terminal region of human HADHA.

UniProt

P40939

RefSeq (Protein):NP_000173.2