HAUSP / USP7 Antibody

SKU: orb1260354

Description

HAUSP / USP7 Antibody

Research Area

,Cancer,Cell Cycle

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 206-1102 of human HAUSP / USP7 (NP_003461.2).
• Target: USP7
• Molecular Weight: Observed: 138kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

USP7, Deubiquitinating enzyme 7, HAUSP, TEF1, Ubiquitin specific protease 7, Ubiquitin thioesterase 7

Western blot analysis of extracts of various cell lines, using HAUSP/USP7 antibody (orb1260354) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunohistochemistry of paraffin-embedded human kidney cancer using HAUSP/USP7 antibody (orb1260354) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse heart using HAUSP/USP7 antibody (orb1260354) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of HeLa cells using HAUSP/USP7 antibody (orb1260354).

Quick Database Links

Gene Symbol

USP7

UniProt

Q93009