HCN2 Antibody: APC

SKU: orb148245

Description

Mouse monoclonal to HCN2 (APC). Hyperpolarization-activated cyclic nucleotide-gated ion channel 2 (HCN2) is an integral membrane protein that helps establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient. Ion channels are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligand- gated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels; each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inward-rectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels. Specifically, hyperpolarization-activated cation channels of the HCN gene family contribute to spontaneous rhythmic activity in both the heart and brain.

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Neuroscience,Epigenetics,Neuroscience | Ion Channels | Cyclic Nucleotide-Gated Ion Channels | cardiovascular System | Heart

Images & Validation

• Tested Applications: ICC, IF, IHC
• Dilution Range: WB (1:1000), IHC (1:1000), ICC/IF (1:100)
• Reactivity: Mouse, Rat
• Application Notes:
  1 µg/ml of SMC-305 was sufficient for detection of HCN2 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: S71
• Immunogen: Fusion protein amino acids 761-863 (cytoplasmic C-terminus) of rat HCN2
• Target: HCN2
• Molecular Weight: 95kDa
• Conjugation: APC

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 95.64mM Phosphate, 2.48mM MES and 2mM EDTA
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BCNG2 antibody, HAC1 antibody, brain cyclic nucleotide gated channel 2 antibody, Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel antibody

Quick Database Links

Gene Symbol

HCN2

UniProt

Q9JKA9

NCBI Gene ID

114244

RefSeq (Protein):NP_446136.1