HDAC1 Antibody, KO Validated

SKU: orb1274572

Description

HDAC1 Antibody, KO Validated

Research Area

,Apoptosis,Cell Cycle,Immunology,Neuroscience,Signal Transduction,Stem Cell

Images & Validation

• Tested Applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:100 - 1:200IF: 1:50 - 1:100IP: 1:50 - 1:100

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 393-482 of human HDAC1 (NP_004955.2).
• Target: HDAC1
• Molecular Weight: Observed: 62kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HDAC1 Antibody: HD1, RPD3, GON-10, RPD3L1, Histone deacetylase 1, HD1

Western blot analysis of extracts of various cell lines, using HDAC1 antibody at 1:1000 dilution.

Western blot analysis of extracts from normal (control) and HDAC1 knockout (KO) 293T cells, using HDAC1 antibody at 1:1000 dilution.

Immunohistochemistry of paraffin-embedded human prostate cancer using HDAC1 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using HDAC1 antibody at dilution of 1:100 (40x lens).

Immunofluorescence analysis of A549 cells using HDAC1 antibody. Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200ug extracts of HT-29 cells, using 3 ug HDAC1 antibody. Western blot was performed from the immunoprecipitate using HDAC1 antibody at a dilition of 1:1000.

Quick Database Links

Gene Symbol

HDAC1

UniProt

Q13547