HDAC1 Rabbit Polyclonal Antibody

SKU: orb704382

Description

HDAC1 Rabbit Polyclonal Antibody

Research Area

Cancer Research, Cardiovascular Research, Epigenetics and Nuclear Signaling, HDACs, Cell Biology

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:400-800, IHC-F=1:400-800, IF=1:100-500
• Reactivity: Rat
• Predicted Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human HDAC1 (341-410/482aa)
• Target: HDAC1
• Molecular Weight: 53 kDa
• Purification: affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DKFZp686H12203; GON 10; HD 1; HD1; HDAC 1; hdac1: histone deacetylase 1; Histone deacetylase 1(HD1); Histone deacetylase 1; RPD3(reduced potassium dependency yeast homolog) like 1; RPD3(reduced potassium dependency); RPD3; RPD3L1; HDAC1_HUMAN.

Paraformaldehyde-fixed, paraffin embedded (rat stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (HDAC1 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (orb704382) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

HDAC1

UniProt

Q13547