HDEL Antibody: FITC

SKU: orb147722

Description

Mouse monoclonal to HDEL (FITC). HSP 70 family comprises four highly conserved proteins, HSP 70, HSC 70, GRP 75 and GRP 78, which serve a variety of roles. They act as molecular chaperones, facilitating the assembly of multi-protein complexes; participate in the translocation of polypeptides across cell membranes and to the nucleus; and aid in the proper folding of nascent polypeptide chains. GRP 78 is localized in the endoplasmic reticulum (ER), where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains. Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually KDEL in animal cells, and HDEL in S. cerevisiae. The 2E7 clone recognizes the C-terminal peptide HDEL, a common version of the endoplasmic reticulum retention signal found in yeast, plant, nematode and other ER proteins. 2E7 specifically stains HDEL proteins in barnyard grass, beet, cotton, mung bean, sorghum and wheat..

Research Area

Product Categories/Products/Antibodies,Product Categories/Products/Antibodies/Primary Antibodies,Cancer | Heat Shock | Cell Signaling | Protein Trafficking

Images & Validation

• Tested Applications: ICC, IF, IHC
• Dilution Range: WB (1:1000), ICC/IF (1:100)
• Reactivity: Drosophila, Plant, Saccharomyces, Yeast
• Application Notes:
  1 µg/ml of SMC-175 was sufficient for detection of HDEL-containing proteins in 10 µg of S. cerevisiae lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone No.: 20000000
• Immunogen: Raised against a synthetic HDEL peptide corresponding to the C-terminus of yeast Bip
• Target: HDEL
• Molecular Weight: 78kDa
• Conjugation: FITC

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 640.91mM DMSO, 136.36mM Ethanolamine, 9.09mM Sodium Bicarbonate in 90.9% PBS
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

H-D-E-L (his-asp-glu-leu) antibody, endoplasmic reticulum antibody, luminal ER protein retention antibody, KDELR1 antibody, Endoplasmic reticulum retention signal antibody

Quick Database Links

Gene Symbol

HDEL