HDEL Antibody

SKU: orb1822459

Description

Mouse Anti-Yeast HDEL Monoclonal IgG2b

Research Area

,Cancer | Heat Shock | Cell Signaling | Protein Trafficking

Images & Validation

• Tested Applications: ICC, IF, WB
• Dilution Range: WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
• Reactivity: Drosophila, Plant, Saccharomyces, Yeast
• Application Notes:
  1 µg/ml of SMC-175 was sufficient for detection of HDEL-containing proteins in 10 µg of S. cerevisiae lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone No.: 2E7
• Immunogen: Raised against a synthetic HDEL peptide corresponding to the C-terminus of yeast Bip
• Target: HDEL
• Molecular Weight: 78kDa
• Conjugation: Unconjugated

Storage & Handling

• Storage: -20°C
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

H-D-E-L (his-asp-glu-leu) Antibody, endoplasmic reticulum Antibody, luminal ER protein retention Antibody, KDELR1 Antibody, Endoplasmic reticulum retention signal Antibody

Quick Database Links

Gene Symbol

HDEL