HER2 Antibody / ErbB2

SKU: orb2643190

Description

This MAb is specific and shows minimal cross-reaction with other members of the EGFR-family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. c-erbB-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.

Images & Validation

• Tested Applications: IHC-P
• Dilution Range: Immunohistochemistry (FFPE): 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG
• Clone No.: SPM172
• Immunogen: A recombinant human HER2 protein fragment within amino acids 311-462 was used as the immunogen for the HER2 antibody.
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This HER2 antibody is available for research use only.
• Disclaimer: For research use only

IHC staining of FFPE human breast carcinoma with HER2 antibody (clone SPM172). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free HER2 antibody (clone SPM172) as confirmation of integrity and purity.

Quick Database Links

UniProt

P04626