Hirudin protein

SKU: orb80174

Description

Recombinant Hirudin is derived from yeast and the polypeptide chain contains 65 amino acids and its Mw is 6979.5 dalton which is identical to natural Hirudin except for the substitution of leucine for isoleucine at the N-terminal end of the molecule and the absence of sulfate group on the tyrosine at position 63.The Recombinant Hirudin is purified by proprietary chromatographic techniques.

Images & Validation

• Tested Applications: FA, HPLC, SDS-PAGE

Key Properties

• Biological Origin: Pichia Pastoris
• Biological Activity: The biological activity is measured using chromogenic assay, unit is defined as the amount of Hirudin that neutralizes unit of the WHO preparation 89/588 of thrombin. The specific ativity was found to be 16,000ATU/mg protein.
• Target: Hirudin
• Endotoxins: Less than 1 EU/μg of rHirudin as determined by LAL method.
• Conjugation: Unconjugated
• MW: 6.7 kDa
• Purity: > 96 % by SDS-PAGE and HPLC analyses.
• Protein Sequence: VVYTDCTESG QNLCLCEGSN VCGQGNKCIL GSDGEKNQCV TGEGTPGPQS HNDGDFEEPE EYL

Storage & Handling

• Storage: Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
• Buffer/Preservatives: Lyophilized from a 0.2 μm filtered solution of 20 mM PBS, pH 7.0, containing 2 % mannitol.
• Concentration: 0.5 mg/ml; 100 ul
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

Hirudin