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Description
Research Area
,Product Categories/Antibodies,Loading control
Images & Validation
−Item 1 of 4
| Tested Applications | IF, IHC, WB |
|---|---|
| Dilution Range | Western Blot 1:1000 - 1:3000 Immunofluorescence 1:200-1:500 Immunohistochemistry ( Paraffin) 1:200-1:400 |
| Reactivity | Human, Mouse, Rabbit |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit-IgG |
| Immunogen | Synthetic peptide / encompassing a sequence within the C-terminus region. |
| Target | Histone H3 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
|---|---|
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Anti-Histone H3 antibody - at 1/2000 dilution, Lysates at 35 μg per lane. This blot was produced using a 15% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294413 overnight at 4°C.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294413 anti-Histone H3 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.
Immunohistochemical of Paraffin-embedded. Sample: mouse duodenum and brain. Histone H3 antibody (orb1294413): 1-300.
Immunohistochemical of Paraffin-embedded. Sample: mouse duodenum; Histone H3 antibody (orb1294413): 1-300.
Quick Database Links
Gene Symbol
Histone H3
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
Histone H3 (orb1294413)
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