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Description
Research Area
,Immunology
Images & Validation
−Item 1 of 4
| Tested Applications | IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 35-285 of human HLA-A (NP_001229687.1). |
| Target | HLA-A |
| Molecular Weight | Observed: 43kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−HLAA, HLA class I histocompatibility antigen, A-1 alpha chain, MHC class I antigen HLA-A heavy chain, leukocyte antigen class I-A
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Western blot analysis of extracts of various cell lines, using HLA-A antibody (orb1258243) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human liver damage using HLA-A antibody (orb1258243) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using HLA-A antibody (orb1258243) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using HLA-A antibody (orb1258243) at dilution of 1:100 (40x lens).
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
HLA-A Antibody (orb1258243)
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