HLA-DR Antibody (MHC II)

SKU: orb317068

Description

This mAb reacts with the HLA-DR antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kDa alpha (heavy) chain and a 28kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense cytoplasmic staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts.

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Product Categories/Products/Antibodies/Primary Antibodies,Immunology,Epigenetics,Infectious Diseases

Images & Validation

• Tested Applications: FACS, IF, IHC-P
• Dilution Range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  Optimal dilution of the HLA-DR antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2a, kappa
• Clone No.: 19-26.1 or MB-26.1
• Immunogen: Raji cells were used as the immunogen for the HLA-DR antibody.
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store the HLA-DR antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This HLA-DR antibody is available for research use only.
• Disclaimer: For research use only

Flow cytometry staining of human Raji cells with HLA-DR antibody (clone 19-26.1 or MB-26.1); Red = isotype control, Blue = HLA-DR antibody.

IHC: Formalin-fixed, paraffin-embedded human tonsil stained with HLA-DR antibody (clone 19-26.1 or MB-26.1).

SDS-PAGE Analysis of Purified, BSA-Free HLA-DR Antibody (clone 19-26.1 or MB-26.1). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

UniProt

P01903