HLA-DRB1 Antibody (N-term)

SKU: orb37144

Description

Peptide affinity purified rabbit polyclonal antibody.

Research Area

Product Categories/Products/Antibodies,Product Categories/Products/Antibodies/Primary Antibodies,Primary Antibodies;Cardiovascular;Immunology;Catalog Updated

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution Range: WB: 1:1000, IHC-P: 1:10-50, FACS: 1:10-50
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB21019
• Immunogen: Synthetic Peptide
• Target: This HLA-D_1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-58 amino acids of human HLA-D_1.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-HLA class II histocompatibility antigen antibody, anti-DRB1-1 beta chain antibody, anti-MHC class I antigen DRB1*1 antibody, anti-DR-1 antibody, anti-DR1 antibody, anti-HLA-DRB1 antibody

Western blot analysis of HLA-DRB1 Antibody (N-term) in WiDr cell line lysates (35 ug/lane). HLA-DRB1 (arrow) was detected using the purified Pab.

Flow cytometric analysis of widr cells using HLA-DRB1 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram) FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of lysates from NCI-H1299, Raji cell line (from left to right), using HLA-DRB1 Antibody (N-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.

Formalin-fixed and paraffin-embedded human brain tissue reacted with HLA-DRB1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Quick Database Links

Gene Symbol

This HLA-D_1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-58 amino acids of human HLA-D_1.

UniProt

P04229