HMGB1 monoclonal antibody (M08), clone 2F6

SKU: orb2292808

Description

Mouse monoclonal antibody raised against a partial recombinant HMGB1.

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a Kappa
• Clone No.: 2F6
• Immunogen: HMGB1 (NP_002119, 1 a.a. ~ 90 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Protein Sequence: MGKGDPKKPRGKMSSYAFFVQTCREEHKKKHPDASVNFSEFSKKCSERWKTMSAKEKGKFEDMAKADKARYEREMKTYIPPKGETKKKFK
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Detection limit for recombinant GST tagged HMGB1 is approximately 0.3 ng/ml as a capture antibody.

HMGB1 monoclonal antibody (M08), clone 2F6 Western Blot analysis of HMGB1 expression in Hela S3 NE.

Immunofluorescence of monoclonal antibody to HMGB1 on HeLa cell. [antibody concentration 10 ug/ml]

Immunoperoxidase of monoclonal antibody to HMGB1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]

Western Blot analysis of HMGB1 expression in transfected 293T cell line by HMGB1 monoclonal antibody (M08), clone 2F6. Lane 1: HMGB1 transfected lysate (25 KDa). Lane 2: Non-transfected lysate.

Western Blot detection against Immunogen (35.64 KDa).

Quick Database Links

RefSeq (Protein):NP_002119