HMGB1 Recombinant Rabbit Monoclonal Antibody

SKU: orb1974619

This product can be custom made

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Description

HMGB1 Recombinant Rabbit Monoclonal Antibody

Research Area

,HMGs,HMG Box,SARS Coronavirus,hmgb1

Images & Validation

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Item 1 of 2

Tested Applications	WB
Dilution Range	WB=1:500-1000
Reactivity	Human, Mouse
Predicted Reactivity	Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Clone No.	10H9
Immunogen	KLH conjugated synthetic peptide derived from human HMGB1
Target	HMGB1
Molecular Weight	25 kDa
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS(pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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High mobility group protein B1; Amphoterin; High mobility group 1; High Mobility Group Box 1; High mobility group protein 1; HMG3; HMGB 1; HMGB-1; Hmgb1 protein; Nonhistone chromosomal protein HMG1; SBP 1; SBP-1; Sulfoglucuronyl carbohydrate binding protein; HMGB1_HUMAN.

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

HMGB1 Recombinant Rabbit Monoclonal Antibody

25 ug total protein per lane of various lysates probed with HMGB1 monoclonal antibody, unconjugated (orb1974619) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Sample: Lane 1: Mouse NIH/3T3 cell lysates, Lane 2: Mouse Lung tissue lysates, Lane 3: Mouse Kidney tissue lysates, Lane 4: Mouse Large intestine tissue lysates, Lane 5: Human Jurkat cell lysates, Lane 6: Human HeLa cell lysates, Lane 7: Human HepG2 cell lysates, Lane 8: Human MOLT4 cell lysates, Lane 9: Human HL-60 cell lysates, Primary: Anti-HMGB1 (orb1974619) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 25 kDa, Observed band size: 30 kDa.

Quick Database Links

Gene Symbol

HMGB1

UniProt

P09429

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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