HNRNPD Antibody (N-term)

SKU: orb107064

Description

Rabbot polyclonal to HNRNPD. This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are nucleic acid binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It localizes to both the nucleus and the cytoplasm. This protein is implicated in the regulation of mRNA stability. Alternative splicing of this gene results in four transcript variants.

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics,Crown Antibodies;Primary Antibodies;Ribonucleoprotein;Viral nucleoprotein

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB: 1:1000, IF/ICC: 1:10-50
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB30287
• Immunogen: Synthetic Peptide
• Target: HNRNPD
• Molecular Weight: 38434
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti HNRNPD antibody, anti AUF1 antibody, anti HNRPD antibody, anti Heterogeneous nuclear ribonucleoprotein D0 antibody, anti AU-rich element RNA-binding protein 1 antibody

HNRNPD Antibody (N-term) western blot analysis in 293T, A431, NCI-H292, Raji cell line lysates (35 ug/lane). This demonstrates the HNRNPD antibody detected the HNRNPD protein (arrow).

All lanes: Anti-HNRNPD Antibody (N-term) at 1:1000 dilution. Lane 1: Hela whole cell lysate. Lane 2: Raji whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 38 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Fluorescent confocal image of Hela cell stained with HNRNPD Antibody (N-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HNRNPD primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). HNRNPD immunoreactivity is localized to Nucleus and Cytoplasm significantly.

Quick Database Links

Gene Symbol

HNRNPD

UniProt

Q14103

RefSeq (Protein):NP_112737.1, NP_112738.1, NP_002129.2, NP_001003810.1