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Description
Research Area
,Other
Images & Validation
−Item 1 of 8
| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 491-730 of human HNRNPM (NP_005959.2). |
| Target | HNRNPM |
| Molecular Weight | Observed: 78kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−HNRPM, DKFZp547H118, HNRNPM, HNRNPM4, HNRPM4, HTGR1, NAGR1, CEAR, HNRPM, hnRNP M
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Western blot analysis of extracts of various cell lines, using HNRNPM antibody (orb1256715) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat brain using HNRNPM antibody (orb1256715) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using HNRNPM antibody (orb1256715) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using HNRNPM antibody (orb1256715) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophageal cancer using HNRNPM antibody (orb1256715) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using HNRNPM antibody (orb1256715) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using HNRNPM antibody (orb1256715) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF7 cells using HNRNPM antibody (orb1256715).
Quick Database Links
Gene Symbol
HNRNPM
UniProt
UniProt Details
− No UniProt data available
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
HNRNPM Antibody (orb1256715)
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