HOP Protein

SKU: orb1714244

Description

Human Recombinant HOP Protein

Research Area

,Cancer | Heat Shock

Images & Validation

• Tested Applications: FA, SDS-PAGE, WB
• Application Notes:
  This product has been certified > 90% pure using SDS PAGE analysis. 4uM SPR-302, when added to 2uM SPR-300 (Aha1)-activated HSP90 (2uM; His-tagged HSP90 beta) in 33mM Hepes pH7.2, 30mM NaCl, 5mM MgCl2, 1mM DTT, 1.5mM ATP in a 100ul reaction at 37 degrees C, eliminated all Aha1-mediated ATPase stimulation as well as intrinsic HSP90 ATPase activity. (This is an enzyme-linked ATP regeneration assay tracking loss of NADH absorbance at 340nm).

Key Properties

• Source: Recombinant
• Expression System: E. coli
• Target: HOP
• Reactivity: Human
• Tag: His tag
• MW: ~63 kDa
• Purity: > 90%
• Protein Sequence: MGHHHHHHMEQVNELKEKGNKALSVGNIDDALQCYSEAIKLDPHNHVLYSNRSAAYAKKGDYQKAYEDGCKTVDLKPDWGKGYSRKAAALEFLNRFEEAKRTYEEGLKHEANNPQLKEGLQNMEARLAERKFMNPFNMPNLYQKLESDPRTRTLLSDPTYRELIEQLRNKPSDLGTKLQDPRIMTTLSVLLGVDLGSMDEEEEIATPPPPPPPKKETKPEPMEEDLPENKKQALKEKELGNDAYKKKDFDTALKHYDKAKELDPTNMTYITNQAAVYFEKGDYNKCRELCEKAIEVGRENREDYRQIAKAYARIGNSYFKEEKYKDAIHFYNKSLAEHRTPDVLKKCQQAEKILKEQERLAYINPDLALEEKNKGNECFQKGDYPQAMKHYTEAIKRNPKDAKLYSNRAACYTKLLEFQLALKDCEECIQLEPTFIKGYTRKAAALEAMKDYTKAMDVYQKALDLDSSCKEAADGYQRCMMAQYNRHDSPEDVKRRAMADPEVQQIMSDPAMRLILEQMQKDPQALSEHLKNPVIAQKIQKLMDVGLIAIR

Storage & Handling

• Storage: -20°C
• Buffer/Preservatives: 25mM HEPES pH 7.2, 50mM NaCl, 1mM DTT, 10% glycerol
• Concentration: Lot/batch specific. See included datasheet.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HSP co chaperone Protein

SDS-PAGE of his-tagged human HOP protein.

Quick Database Links

Gene Symbol

HOP

UniProt

P31948