Hsp 60 Antibody (N-term)

SKU: orb1429535

Description

Rabbit polyclonal antibody to Hsp 60.

Research Area

Product Categories/Products/Antibodies,Crown Antibodies;Primary Antibodies;Signal Transduction;Stress response

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB: 1:2000, WB: 1:2000, WB: 1:1000, IHC-P: 1:50~100, IHC-P: 1:25
• Reactivity: Human, Mouse

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB19532
• Immunogen: Synthetic Peptide
• Target: HSPA14
• Molecular Weight: 54794
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Heat shock 70 kDa protein 14, HSP70-like protein 1, Heat shock protein HSP60, HSPA14, HSP60, HSP70L1

All lanes: Anti-Hsp 60 Antibody (N-term) at 1:2000 dilution. Lane 1: K562 whole cell lysate. Lane 2: 293T/17 whole cell lysate. Lane 3: Jurkat whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 55 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-Hsp 60 Antibody (N-term) at 1:2000 dilution. Lane 1: Raji whole cell lysates. Lane 2: K562 whole cell lysates. Lane 3: Jurkat whole cell lysates. Lane 4: Hela whole cell lysates.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 55 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human lung carcinoma with Hsp 60 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Staining Hsp 60 in human pancreas sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Western blot analysis of Hsp 60 Antibody (N-term) in mouse lung tissue lysates (35 ug/lane). Hsp (arrow) was detected using the purified Pab.

Quick Database Links

Gene Symbol

HSPA14

UniProt

Q0VDF9

RefSeq (Protein):NP_057383.2