HSP25/HSP27 Antibody

SKU: orb1822500

Description

Mouse Anti-Human HSP25/HSP27 Monoclonal IgG1 Kappa

Research Area

,Cell Signaling | Protein Trafficking | Chaperone Proteins | Cell Signaling | Cytoskeleton | Microfilaments | Actin | Actin Assembly | Cell Signaling | Cytoskeleton | Microtubules | Cancer | Tumor Biomarkers | Cardiovascular System | Atherosclerosis | Cardiovascular System | Heart | Contractility | Cancer | Heat Shock

Images & Validation

• Tested Applications: FACS, FC, ICC, IF, IHC, IP, WB
• Dilution Range: WB (1:5000), ICC/IF (1:200), IHC (1:100); optimal dilutions for assays should be determined by the user.
• Reactivity: Bovine, Canine, Guinea pig, Hamster, Human, Mouse, Rat, Sheep
• Application Notes:
  A 1:5000 dilution of SMC-114 was sufficient for detection of HSP27 in 20 µg of HeLa cell lysate by ECL immunoblot analysis.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1 Kappa
• Clone No.: 8A7
• Immunogen: Human HSP27 peptide
• Target: HSP27
• Molecular Weight: 27kDa
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HSPB1 Antibody, Heat shock protein beta-1 Antibody, HSPB1 Antibody, DMPK-binding protein Antibody, MKBP Antibody

Immunohistochemistry analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7. Tissue: embryo somites. Species: Rat. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody at 1:1000. Secondary Antibody: FITC Goat Anti-Mouse (green). Counterstain: Rhodamine-phalloidin labeled actin (red). Investigation at 11 days gestation.

Immunohistochemistry analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody at 1:5000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 μl for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.

Immunohistochemistry analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.

Immunohistochemistry analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.

Western Blot analysis of Rat Lung tissue lysates showing detection of Hsp27 protein using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7. Load: 15 μg. Block: 5% blocking solution. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse: HRP for 1 hour at RT.

Quick Database Links

Gene Symbol

HSP27

UniProt

P04792

NCBI Gene ID

3315

RefSeq (Protein):NP_001532.1