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HSP25/HSP27 Antibody

SKU: orb1822500

Description

Mouse Anti-Human HSP25/HSP27 Monoclonal IgG1 Kappa

Research Area

,Cell Signaling | Protein Trafficking | Chaperone Proteins | Cell Signaling | Cytoskeleton | Microfilaments | Actin | Actin Assembly | Cell Signaling | Cytoskeleton | Microtubules | Cancer | Tumor Biomarkers | Cardiovascular System | Atherosclerosis | Cardiovascular System | Heart | Contractility | Cancer | Heat Shock

Images & Validation

Tested ApplicationsFACS, FC, ICC, IF, IHC, IP, WB
Dilution RangeWB (1:5000), ICC/IF (1:200), IHC (1:100); optimal dilutions for assays should be determined by the user.
ReactivityBovine, Canine, Guinea pig, Hamster, Human, Mouse, Rat, Sheep
Application Notes
A 1:5000 dilution of SMC-114 was sufficient for detection of HSP27 in 20 µg of HeLa cell lysate by ECL immunoblot analysis.

Key Properties

HostMouse
ClonalityMonoclonal
IsotypeIgG1 Kappa
Clone No.8A7
ImmunogenHuman HSP27 peptide
TargetHSP27
Molecular Weight27kDa
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPBS, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
Concentration1 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

HSPB1 Antibody, Heat shock protein beta-1 Antibody, HSPB1 Antibody, DMPK-binding protein Antibody, MKBP Antibody

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Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
FACS
Fluorescence-Activated Cell Sorting (FC, Flow cytometry)
View Protocol
FC
Flow Cytometry
View Protocol
IF
Immunofluorescence
View Protocol
ICC
Immunocytochemistry
View Protocol
IP
Immunoprecipitation
View Protocol

Available Sizes

Select a size below

100 μg
$ 470.00
Choose Conjugation or Carrier Free Version
Free Secondary Antibody (20 ul)0/0

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DispatchUsually dispatched within 2-3 weeks
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