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Description
Research Area
Product Categories/Products/Antibodies,Product Categories/Research Area,Cardiovascular Disease,Neuroscience,Epigenetics,Infectious Diseases,Primary Antibodies;Cancer;Cardiovascular;Cell Biology;Microbiology;Signal Transduction;Stress response
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| Tested Applications | IHC-P, WB |
|---|---|
| Dilution Range | WB: 1:1000, IHC-P: 1:50-100 |
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Clone No. | RB19530 |
| Immunogen | Synthetic Peptide |
| Target | HSPB1 |
| Molecular Weight | 22783 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Anti-Heat shock protein beta-1 antibody, anti-HspB1 antibody, anti-Heat shock 27 kDa protein antibody, anti-HSP 27 antibody, anti-Stress-responsive protein 27 antibody, anti-SRP27 antibody, anti-Estrogen-regulated 24 kDa protein antibody, anti-28 kDa heat shock protein antibody, anti-HSPB1 antibody, anti-HSP27 antibody
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Western blot analysis of HSP27 antibody (Center) in MCF-7 cell line lysates (35 ug/lane). HSP27 (arrow) was detected using the purified Pab.
Hsp 27 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human Lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Hsp 27 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001531.1 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
HSP27 Antibody (Center) (orb37581)
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