HSP27 Antibody [SPM252]

SKU: orb1251935

Description

HSP27 Antibody [SPM252]

Research Area

,Cancer,Cell Cycle,Signal Transduction

Images & Validation

• Tested Applications: FC, IF, IHC, WB
• Reactivity: Gallus, Human, Mouse, Primate, Rat, Sheep
• Application Notes:
  Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlWestern Blotting (0.25-0.5 ug/ml)Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The optimal dilution of the HSP27 antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone No.: SPM252
• Immunogen: Partially purified HSP27 protein from the human breast cancer cell line MCF-7 was used as the immunogen for this HSP27 antibody.
• Target: HSPB1
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Heat shock protein beta-1, HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, HSP 27, Stress-responsive protein 27, SRP27, HSPB1, HSP27, HSP28

Formalin-fixed, paraffin-embedded human breast carcinoma stained with HSP27 antibody (SPM252)

Formalin-fixed, paraffin-embedded human breast carcinoma stained with HSP27 antibody (SPM252)

Quick Database Links

Gene Symbol

HSPB1

UniProt

P04792