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−Item 1 of 6
| Tested Applications | FC, ICC, IHC, WB |
|---|---|
| Dilution Range | WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; ICC: 1/200 - 1/1000; FCM: 1/200 - 1/400; ELISA: 1/10000 |
| Reactivity | Human, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | 5D7C6 |
| Immunogen | Purified recombinant fragment of human HSP27 expressed in E. Coli. |
| Target | HSP27 |
| Molecular Weight | 27kDa |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Ascitic fluid containing 0.03% sodium azide. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; DKFZp586P1322; HSPB1
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Immunohistochemical analysis of paraffin-embedded brain tissues (left) and esophageal cancer tissues (right) using HSP27 antibody
Western blot analysis of Hela (Lane1), A549 (Lane2), Jurkat (Lane3), A431 (Lane4), HEK293(Lane5), HepG2 (Lane6) and PC-12 (La
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
ICC
Immunocytochemistry
HSP27 (orb69427)
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