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Description
Research Area
Images & Validation
−| Tested Applications | ELISA, IHC-P, WB |
|---|---|
| Dilution Range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Reactivity | Human, Monkey |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human HSP27 around the phosphorylation site of Ser15. AA range:5-54 |
| Molecular Weight | 26 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Phospho-HSP27 (Ser15) Rabbit pAb [orb2715054]
IHC-P, IP, WB
Human, Mouse, Rat
Polyclonal
Unconjugated
100 μl, 50 μl

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Western Blot analysis of various cells using Phospho-HSP27 (S15) Polyclonal Antibody.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using HSP27 (Phospho-Ser15) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from HeLa cells treated with UV, using HSP27 (Phospho-Ser15) Antibody. The lane on the left is blocked with the phospho peptide.
Protocol Information
HSP27 (phospho Ser15) rabbit pAb (orb764205)
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