HSP27 (phospho Ser78) rabbit pAb

SKU: orb764206

This product can be custom made

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Description

Rabbit polyclonal antibody to HSP27 (phospho-Ser78)

Research Area

Product Categories/Antibodies/Primary Antibodies,Product Categories/Small Molecules,Product Categories/Antibodies

Images & Validation

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Item 1 of 3

Tested Applications	ELISA, IHC-P, WB
Dilution Range	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Reactivity	Human, Mouse, Rat

Key Properties

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Clonality	Polyclonal
Isotype	IgG
Immunogen	The antiserum was produced against synthesized peptide derived from human HSP27 around the phosphorylation site of Ser78. AA range:45-94
Molecular Weight	27
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Anti-HSPB1 antibody, anti-HSP27 antibody, anti-HSP28 antibody, anti-Heat shock protein beta-1 antibody, anti-HspB1 antibody, anti-28 kDa heat shock protein antibody, anti-Estrogen-regulated 24 kDa protein antibody, anti-Heat shock 27 kDa protein antibody, anti-HSP 27 antibody, anti-Stress-responsive protein 27 antibody, anti-SRP27 antibody

Quality Guarantee

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Western Blot analysis of various cells using Phospho-HSP27 (S78) Polyclonal Antibody.

Western blot analysis of lysates from HeLa cells treated with Ca2 + , using HSP27 (Phospho-Ser78) Antibody. The lane on the left is blocked with the phospho peptide.

Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).