HSP40, Hdj1 Antibody: FITC

SKU: orb147280

Description

Mouse monoclonal antibody to Hsp40 conjugated to PerCP.

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics,Cancer | Heat Shock | Cell Signaling | Protein Trafficking | Chaperone Proteins

Images & Validation

• Tested Applications: ICC, IF, IHC
• Dilution Range: WB (1:2000), ICC/IF (1:100)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  0.5 µg/ml of SMC-145 was sufficient for detection of HSP40 (HDJ1) in 15 µg of HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 3B9.E6
• Immunogen: Recombinant Protein HSP40 (Hdj1)
• Target: HSP40
• Molecular Weight: 40kDa
• Conjugation: FITC

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 640.91mM DMSO, 136.36mM Ethanolamine, and 9.09mM Sodium Bicarbonate in 90.9% PBS
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DNAJ1 antibody, NDAJB1 antibody, HDJ1 antibody, HSP40 antibody, HSPF1 antibody, DnaJ homolog subfamily B member 1 antibody, Dna J protein homolog 1 antibody, Heat shock 40 kDa protein 1 antibody, HSP40 antibody, heat shock protein 40 antibody, Human DnaJ protein 1 antibody, hDj-1 antibody

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp40 Monoclonal Antibody, Clone 3B9.E6. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-Hsp40 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.

Western Blot analysis of Human Cell lysates showing detection of Hsp40 protein using Mouse Anti-Hsp40 Monoclonal Antibody, Clone 3B9.E6. Load: 15 μg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp40 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Quick Database Links

Gene Symbol

HSP40

UniProt

P25685

NCBI Gene ID

3337

RefSeq (Protein):NP_006136.1