HSP40, YDJ1 Antibody: HRP

SKU: orb147332

Description

Mouse monoclonal to Hsp40, YDJ1 (HRP). Human Hsp40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain. There are two main types of Hsp40; type 1 DNAJ proteins including HDJ2 and yeast YdjI; type II includes yeast Sis1 and human Hdj1. Whereas type I possesses a zinc finger domain which helps in the function of protein folding. , type II does not. Members of the Hsp40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. Hsp40 stimulates the ATPase activity of Hsp70 which in turn causes conformational changes of the unfolded proteins . The Hsp40-Hsp70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1..

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics,Cancer | Heat Shock | Cell Signaling | Protein Trafficking | Chaperone Proteins

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: WB (1:2000)
• Reactivity: Yeast
• Application Notes:
  0.5 µg/ml of SMC-150 was sufficient for detection of 50 ng YDJ1 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1 Kappa
• Clone No.: 1G10.H8
• Immunogen: Full length protein yeast HSP40 (YDJ1)
• Target: HSP40
• Molecular Weight: 40kDa
• Conjugation: HRP

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide and 0.23mM Citrate in dH2O
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DNAJA2 antibody, CPR3 antibody, HIRIP4 antibody, DNAJ antibody, DNJ3 antibody, DJ3 antibody, RDJ2 antibody, HIRa interacting protein4 antibody

Western Blot analysis of Yeast Cell lysates showing detection of Hsp40 protein using Mouse Anti-Hsp40 Monoclonal Antibody, Clone 1G10.H8. Load: 15 μg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp40 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Quick Database Links

Gene Symbol

HSP40

UniProt

P25491

NCBI Gene ID

855661

RefSeq (Protein):NP_014335.1