Hsp60 Antibody

SKU: orb1261747

Description

Hsp60 Antibody

Research Area

,Apoptosis,Cell Cycle,Signal Transduction

Images & Validation

• Tested Applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100IP: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 27-240 of human Hsp60 (NP_002147.2).
• Target: HSPD1
• Molecular Weight: Observed: 61kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

60 kDa heat shock protein, mitochondrial, 60 kDa chaperonin, Chaperonin 60, CPN60, Heat shock protein 60, HSP-60, Hsp60, HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein, HSPD1, HSP60

Western blot analysis of extracts of various cell lines, using Hsp60 antibody at 1:1000 dilution.

Immunohistochemistry of paraffin-embedded rat kidney using Hsp60 antibody at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using Hsp60 antibody. Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug Hsp60 antibody. Western blot was performed from the immunoprecipitate using Hsp60 antibody at a dilution of 1:1000.

Quick Database Links

Gene Symbol

HSPD1

UniProt

P10809