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Description
Research Area
,Primary Antibodies;Signal Transduction;Viral process;Host-virus interaction;Immune system process;Mitochondrion;Stress response
Images & Validation
−Item 1 of 2
| Tested Applications | WB |
|---|---|
| Dilution Range | IHC: 1:1000, WB: 1:40000 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Hamster |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | 396-430 aa |
| Target | This Hsp60 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 396-430 amino acids from human Hsp60. |
| Molecular Weight | 61055 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−60 kDa heat shock protein, mitochondrial, 60 kDa chaperonin, Chaperonin 60, CPN60, Heat shock protein 60, HSP-60, Hsp60, HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein, HSPD1, HSP60
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Immunohistochemical analysis of paraffin-embedded Human liver section using Pink1. Diluted at 1:1000 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
All lanes: Anti-Hsp60 Antibody at 1:40000 dilution. Lane 1: Hela whole cell lysate. Lane 2: A431 whole cell lysate. Lane 3: A549 whole cell lysate. Lane 4: HepG2 whole cell lysate. Lane 5: NIH/3T3 whole cell lysate. Lane 6: C2C12 whole cell lysate. Lane 7: PC-12 whole cell lysate. Lane 8: C6 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 60 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Quick Database Links
Gene Symbol
This Hsp60 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 396-430 amino acids from human Hsp60.
UniProt
UniProt Details
− No UniProt data available
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Hsp60 Antibody (orb1787838)
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