HSP60 Antibody

SKU: orb2636689

Description

Recognizes a 60kDa protein, identified as the heat shock protein 60 (hsp60). Its epitope is localized between aa 383-447 of human hsp60. A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsp s), more appropriately called as stress response proteins (srp s). hsp60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial hsp60, but also against its mammalian homolog. Clone HSPD1/6498R, unlike clone LK2, recognizes only the mammalian (not bacterial) hsp60 and is useful in distinguishing hsp60 from mammals and bacteria.

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Optimal dilution of the HSP60/GROEL antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: Rabbit IgG, kappa
• Clone No.: HSPD1/6498R
• Immunogen: Recombinant human full-length HSP60 protein was used as the immunogen for the HSP60/GROEL antibody. Its epitope is localized between amino acids 383-447 of human hsp60.
• Purity: Protein A/G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This HSP60/GROEL antibody is available for research use only.
• Disclaimer: For research use only

Western blot testing of human 1) Hep2 and 2) HeLa cell lysate using HSP60/GROEL antibody (clone HSPD1/6498R). Predicted molecular weight: ~60 kDa.

Western blot testing of human Hep2 cell lysate using HSP60/GROEL antibody (clone HSPD1/6498R). Predicted molecular weight: ~60 kDa.

IHC staining of FFPE human liver tissue with HSP60/GROEL antibody (clone HSPD1/6498R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Quick Database Links

UniProt

P10809