HSP60 Antibody

SKU: orb2637740

Description

This antibody recognizes a 60kDa protein, identified as the heat shock protein 60 (hsp60). The antibody's epitope is localized between amino acids 383-419 of human HSP60. A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsp's), more appropriately called as stress response proteins (srp's). HSP60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial HSP60, but also against its mammalian homolog. Clone LK2 antibody, unlike LK1 antibody, recognizes both mammalian and bacterial HSP60.

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Reactivity: Human
• Application Notes:
  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: LK2
• Immunogen: Recombinant human HSP60 protein was used as the immunogen for this antibody.
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This HSP60 antibody is available for research use only.
• Disclaimer: For research use only

IHC staining of FFPE human liver tissue with HSP60 antibody (clone LK2). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Immunofluorescent staining of MeOH-fixed human HeLa cells with HSP60 antibody (clone LK2, green) and Reddot nuclear stain (red).

Western blot testing of Hep-2 cell lysate with HSP60 antibody (clone LK2).

SDS-PAGE analysis of purified, BSA-free HSP60 antibody (clone LK2) as confirmation of integrity and purity.

Quick Database Links

NCBI Gene ID

3329