HSP60 Antibody: PerCP

SKU: orb146875

Description

Mouse monoclonal to Hsp60 (PerCP). In both prokaryotic and eukaryotic cells, the misfolding In both prokaryotic and eukaryotic cells, the misfolding...

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Cancer,Neuroscience,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics,Infectious Diseases,Cancer | Heat Shock | Cell Signaling | Protein Trafficking | Chaperone Proteins | Tags and Cell Markers | Organelle Markers

Images & Validation

• Tested Applications: ELISA, ICC, IF, IHC, WB
• Dilution Range: WB (1:4000), IHC (1:100)
• Reactivity: Bacteria, Bovine, Canine, E. coli, Fish, Gallus, Guinea pig, H. pylori, Hamster, Human, Insect, Monkey, Mouse, Other, Plant, Porcine, Rabbit, Rat, Yeast
• Application Notes:
  0.25 µg/ml of SMC-111 was sufficient for detection of HSP60 in 10 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using goat anti-mouse IgG as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: LK2
• Immunogen: Recombinant human HSP60
• Target: HSP60
• Molecular Weight: 60kDa
• Conjugation: PerCP

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 95.64mM Phosphate, 2.48mM MES and 2mM EDTA
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CPN60 antibody, GROEL antibody, HLD4 antibody, HSP 60 antibody, HSP65 antibody, HSPD1 antibody, HuCHA60 antibody, SPG 13 antibody

Immunohistochemistry analysis using Mouse Anti-Hsp60 Monoclonal Antibody, Clone LK-2. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp60 Monoclonal Antibody at 1:100000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 μl for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.

Immunohistochemistry analysis using Mouse Anti-Hsp60 Monoclonal Antibody, Clone LK-2. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp60 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Cytoplasmic Staining.

Immunohistochemistry analysis using Mouse Anti-HSP60 Monoclonal Antibody, Clone LK2. Tissue: Kidney. Species: Human. Fixation: Formalin Fixed, Paraffin Embedded. Primary Antibody: Mouse Anti-HSP60 Monoclonal Antibody.

Western Blot analysis of Human Heat Shocked cervical cancer cell line (HeLa) lysate showing detection of Hsp60 protein using Mouse Anti-Hsp60 Monoclonal Antibody, Clone LK-2. Load: 15 μg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp60 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Quick Database Links

Gene Symbol

HSP60

UniProt

P10809

NCBI Gene ID

3329

RefSeq (Protein):NP_002147.2