HSP60 Antibody [SPM253]

SKU: orb1252527

Description

HSP60 Antibody [SPM253]

Research Area

,Signal Transduction

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Reactivity: Bovine, Canine, Drosophila, Frog, Gallus, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep
• Application Notes:
  Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlWestern blot: 0.25-0.5 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the anti-HSP60 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone No.: SPM253
• Immunogen: Recombinant human protein was used as the immunogen for the anti-HSP60 antibody.
• Target: HSPD1
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

60 kDa heat shock protein, mitochondrial, 60 kDa chaperonin, Chaperonin 60, CPN60, Heat shock protein 60, HSP-60, Hsp60, HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein, HSPD1, HSP60

Formalin-fixed, paraffin-embedded human bladder carcinoma stained with anti-HSP60 antibody (SPM253)

Formalin-fixed, paraffin-embedded human pancreas stained with anti-HSP60 antibody (SPM253).

Quick Database Links

Gene Symbol

HSPD1

UniProt

P10809